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The balanced sampling design is such that it will also initiate a “natural experiment” that over time will investigate the effect of no-take zones on fish diversity and abundance, benthic community structure, and trophic complexity. This will also enable the determination of the value of the MPA for fisheries sustainability support and resilience to disturbances caused by extrinsic factors such as pollution or climate change. Additionally, this study will assess whether the zonation plan provides for representation of all reef and canyon types within each zone or if there is unbalanced coverage for certain reefs and in certain zones. This is important to assess at the initiation of the MPA, especially to ensure that the interpretation of monitoring results in future, which compare ecosystems and fish on reefs and canyons in the mesophotic zone are not flawed by natural differences from the outset due to the selection of zone boundaries. 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